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Title: Plasmodium vivax gametocyte protein Pvs230 is a transmission-blocking vaccine candidate
Authors: Mayumi Tachibana
Chiho Sato
Hitoshi Otsuki
Jetsumon Sattabongkot
Osamu Kaneko
Motomi Torii
Takafumi Tsuboi
Ehime University
Tottori University
Armed Forces Research Institute of Medical Sciences, Thailand
Nagasaki University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine;Veterinary
Issue Date: 27-Feb-2012
Citation: Vaccine. Vol.30, No.10 (2012), 1807-1812
Abstract: The malaria transmission-blocking vaccine (TBV) aims to interfere the development of malaria parasite in the mosquito and prevent further transmission in the community. So far only two TBV candidates have been identified in Plasmodium vivax; ookinete surface proteins Pvs25 and Pvs28. The pvs230 (PVX_003905) is reported as an ortholog of Pfs230, a gametocyte/gamete stage TBV candidate in Plasmodium falciparum, however its candidacy for TBV has never been tested. Therefore here, we have investigated whether Pvs230 can be a TBV candidate using P. vivax samples obtained from Thailand. The mouse antiserum raised against the plasmid expressing CRDs I-IV of Pvs230 detected Pvs230 protein in the lysate of P. vivax gametocyte in western blot analysis under non-reducing condition. From the localization of Pvs230 on the outer most regions of gametocyte in the immunofluorescence assay, it appears that Pvs230 is localized on the surface of gametes. Importantly, the anti-Pvs230 mouse serum significantly reduced the number of P. vivax oocysts developed in the mosquito midgut. Moreover, the polymorphism in Pvs230 CRDs I-IV is limited suggesting that it may not be an impediment for the utilization of Pvs230 as an effective TBV candidate. In conclusion, our results show that Pvs230 is a transmission-blocking vaccine candidate of P. vivax. © 2012 Elsevier Ltd.
ISSN: 18732518
Appears in Collections:Scopus 2011-2015

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