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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13811
Title: Role of myocyte enhancing factor 2B in epithelial myofibroblast transition of human gingival keratinocytes
Authors: Qiang Sun
Apsorn Sattayakhom
Johannes Backs
Wolfgang Stremmel
Walee Chamulitrat
Forschungsgruppen
Universitatsklinikum Mannheim
Mahidol University
Department of Internal Medicine III (Cardiology)
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Feb-2012
Citation: Experimental Biology and Medicine. Vol.237, No.2 (2012), 178-185
Abstract: It has recently emerged that the myogenic contribution of the epithelial mesenchymal transition plays a role in neoplastic invasion and metastasis. Myocyte enhancing factor 2B (MEF2B) is the only MEF2 isoform expressed during early embryonic development, and is herein proposed to transactivate the downstream target proteins of the epithelial myofibroblast transition (EMyT). We have previously generated eight preneoplastic cell lines with spindle and cobblestone morphology from human gingival mucosal keratinocytes immortalized by E6/E7 of human papillomavirus type 16. Spindle cells formed tubulogenic morphogenesis on Matrigel and exhibited contractility, anchorage-independent growth and invasiveness to a greater extent than cobblestone cells. Expression of MEF2B mRNA and myofibroblast proteins was higher in spindle cells compared with cobblestone cells. Epidermal growth factor (EGF) treatment of cobblestone cells also induced expression of these genes. Knockdown of MEF2B in a cobblestone cell line abolished EGF-induced upregulation of MEF2, vimentin and non-muscle caldesmon proteins, but enhanced basal expression of mesenchymal vimentin and fibronectin. Differential regulation of intermediate filaments revealed an unrecognized role of MEF2B in myogenic transformation of the epithelial to a myofibroblast phenotype, which occurs as epithelioid variants in some soft tissue sarcomas. © 2012 by the Society for Experimental Biology and Medicine.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84857339916&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13811
ISSN: 15353699
15353702
Appears in Collections:Scopus 2011-2015

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