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Title: Characterization of mucus-associated proteins from abalone (Haliotis) - Candidates for chemical signaling
Authors: Chitraporn Kuanpradit
Michael J. Stewart
Patrick S. York
Bernard M. Degnan
Prasert Sobhon
Peter J. Hanna
Jittipan Chavadej
Scott F. Cummins
Srinakharinwirot University
Mahidol University
University of Tasmania
University of Queensland
Deakin University
University of the Sunshine Coast
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Feb-2012
Citation: FEBS Journal. Vol.279, No.3 (2012), 437-450
Abstract: Living in groups is a widespread phenomenon in the animal kingdom. For free-spawning aquatic animals, such as the abalone (Haliotis), being in the close proximity to potential mating partners enhances reproductive success. In this study, we investigated whether chemical cues could be present in abalone mucus that enable species-specific aggregation. A comparative MS analysis of mucus obtained from trailing or fixed stationary Haliotis asinina, and from seawater surrounding aggregations, indicated that water-soluble biomolecules are present and that these can stimulate sensory activity in conspecifics. Purified extracts of trail mucus contain at least three small proteins [termed H. asinina mucus-associated proteins (Has-MAPs)-1-3], which readily diffuse into the surrounding seawater and evoke a robust cephalic tentacle response in conspecifics. Mature Has-MAP-1 is approximately 9.9 kDa in size, and has a glycine-rich N-terminal region. Has-MAP-2 is approximately 6.2 kDa in size, and has similarities to schistosomin, a protein that is known to play a role in mollusc reproduction. The mature Has-MAP-3 is approximately 12.5 kDa in size, and could only be identified within trail mucus of animals outside of the reproductive season. All three Has-MAP genes are expressed at high levels within secretory cells of the juvenile abalone posterior pedal gland, consistent with a role in scent marking. We infer from these results that abalone mucus-associated proteins are candidate chemical cues that could provide informational cues to conspecifics living in close proximity and, given their apparent stability and hydrophilicity, animals further afield. © 2011 FEBS. No claim to original Australian government works.
ISSN: 17424658
Appears in Collections:Scopus 2011-2015

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