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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13824
Title: CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae
Authors: Suthee Benjaphokee
Preeyaporn Koedrith
Choowong Auesukaree
Thipa Asvarak
Minetaka Sugiyama
Yoshinobu Kaneko
Chuenchit Boonchird
Satoshi Harashima
Osaka University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering
Issue Date: 15-Jan-2012
Citation: New Biotechnology. Vol.29, No.2 (2012), 166-176
Abstract: Use of thermotolerant strains is a promising way to reduce the cost of maintaining optimum temperatures in the fermentation process. Here we investigated genetically a Saccharomyces cerevisiae strain showing a high-temperature (41°C) growth (Htg + ) phenotype and the result suggested that the Htg + phenotype of this Htg + strain is dominant and under the control of most probably six genes, designated HTG1 to HTG6. As compared with a Htg - strain, the Htg + strain showed a higher survival rate after exposure to heat shock at 48°C. Moreover, the Htg + strain exhibited a significantly high content of trehalose when cultured at high temperature and stronger resistance to Congo Red, an agent that interferes with cell wall construction. These results suggest that a strengthened cell wall in combination with increased trehalose accumulation can support growth at high temperature. The gene CDC19, encoding pyruvate kinase, was cloned as the HTG2 gene. The CDC19 allele from the Htg + strain possessed five base changes in its upstream region, and two base changes resulting in silent mutations in its coding region. Interestingly, the latter base changes are probably responsible for the increased pyruvate kinase activity of the Htg + strain. The possible mechanism leading to this increased activity and to the Htg + phenotype, which may lead to the activation of energy metabolism to maintain cellular homeostasis, is discussed. © 2011 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84855810623&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13824
ISSN: 18764347
18716784
Appears in Collections:Scopus 2011-2015

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