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Title: Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development
Authors: Parichat Lapcharoen
Narumon Komalamisra
Yupha Rongsriyam
Voranuch Wangsuphachart
Paron Dekumyoy
Jetsumon Prachumsri
Mayur K. Kajla
Susan M. Paskewitz
Mahidol University
Armed Forces Research Institute of Medical Sciences, Thailand
University of Wisconsin Madison
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jan-2012
Citation: Developmental and Comparative Immunology. Vol.36, No.1 (2012), 104-111
Abstract: A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623. bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4. kDa and theoretical pI of 8.45. Six cysteine residues and a potential calcium binding motif that are present i n AdLys c-1 are highly conserved relative to those of c-type lysozymes found in other insects. RT-PCR analysis of the AdLys c-1 transcript revealed its presence at high levels in the salivary glands both in larval and adult stages and in the larval caecum. dsRNA mediated gene knockdown experiments were conducted to examine the potential role of this lysozyme during Plasmodium berghei infection. Silencing of AdLys c-1 resulted in a significant reduction in the number of oocysts as compared to control dsGFP injected mosquitoes. © 2011 Elsevier Ltd.
ISSN: 0145305X
Appears in Collections:Scopus 2011-2015

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