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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/13920
Title: Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics
Authors: Haik Chosrowjan
Seiji Taniguchi
Thanyaporn Wongnate
Jeerus Sucharitakul
Pimchai Chaiyen
Fumio Tanaka
Institute for Laser Technology
Mahidol University
Chulalongkorn University
Keywords: Chemical Engineering;Chemistry;Physics and Astronomy
Issue Date: 15-Apr-2012
Citation: Journal of Photochemistry and Photobiology A: Chemistry. Vol.234, (2012), 44-48
Abstract: Ultrafast fluorescence dynamics of flavin adenine dinucleotide (FAD) in wild type pyranose 2-oxidase (P2O) has been investigated in solution by means of fluorescence up-conversion method. Fluorescence decays were well described by two-exponential model function. Fluorescence lifetimes were τ 1 ∼ 110 fs and τ 2 ∼ 360 ps, respectively. The ( τ2 / τ1 ) ratio (∼3200) was extraordinary high compared to other flavoproteins without subunit structure. The heterogeneous distribution of emission lifetimes were elucidated in terms of two different conformers of P2O; conformer 1 with τ 1 and conformer 2 with τ 2 . Emission peaks of conformer 1 and conformer 2 were determined to be at ∼540 nm and 510 nm, respectively, using transient spectral reconstruction procedure. Using dynamics analysis by Kakitani and Mataga (KM) theory, both quenching processes were ascribed to photoinduced electron transfer (ET) reactions mainly from Trp168 to the excited isoalloxazine (Iso*) in different protein tetramers having different static dielectric constants (ε 1 ∼ 3.25 for conformer 1 and ε 2 ∼ 5.93 for conformer 2). The quaternary structure seems to be responsible for the observed conformational heterogeneity. © 2012 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84859543802&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/13920
ISSN: 10106030
Appears in Collections:Scopus 2011-2015

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