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|Title:||Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor|
Thailand National Center for Genetic Engineering and Biotechnology
|Citation:||Chemistry Letters. Vol.41, No.12 (2012), 1666-1668|
|Abstract:||A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 12Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a highthroughput manner with high sensitivity and selectivity. © 2012 The Chemical Society of Japan.|
|Appears in Collections:||Scopus 2011-2015|
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