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Title: Label-free fluorescent detection of loop-mediated isothermal amplification of nucleic acid using pyrophosphate-selective xanthene-based Zn(II)-coordination chemosensor
Authors: Ekkachai Kittiloespaisan
Akio Ojida
Itaru Hamachi
Yortyot Seetang-Nun
Wansika Kiatpathomchai
Jirarut Wongkongkatep
Mahidol University
Kyushu University
Kyoto University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Chemistry
Issue Date: 1-Dec-2012
Citation: Chemistry Letters. Vol.41, No.12 (2012), 1666-1668
Abstract: A label-free fluorescent detection method for the loopmediated isothermal amplification (LAMP) of nucleic acids was first developed by using the pyrophosphate-selective xanthenebased Zn(II)-coordination chemosensor 12Zn(II). In this method, the production of inorganic pyrophosphate (PPi), a by-product of the LAMP reaction, was detected by 12Zn(II) with a large fluorescence enhancement. On combination with the fluorescent plate reader technique, this method allows us to detect the shrimp white spot syndrome virus in a highthroughput manner with high sensitivity and selectivity. © 2012 The Chemical Society of Japan.
ISSN: 13480715
Appears in Collections:Scopus 2011-2015

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