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|Title:||Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe|
James Cook University, Australia
Thailand National Center for Genetic Engineering and Biotechnology
|Keywords:||Immunology and Microbiology|
|Citation:||Journal of Virological Methods. Vol.186, No.1-2 (2012), 36-42|
|Abstract:||Salt-induced self-aggregation of gold nanoparticles (AuNP) carrying unisense ssDNA probes can be prevented specifically by complementary DNA. Loop-mediated isothermal amplification (LAMP) can amplify DNA rapidly. Here, the two techniques were combined to detect yellow head virus (YHV). The LAMP-AuNP method required 60. min for LAMP and 5. min for hybridization of LAMP products to an AuNP-labeled ssDNA probe followed by salt induced probe-particle aggregation to visualize color development. The detection sensitivity of the method was comparable to that of the commercial IQ2000™ nested RT-PCR but only required ∼65. min to produce a result, and did not cross-detect other shrimp viruses. As the LAMP-AuNP protocol only requires a heating block, it offers opportunities for rapid detection of YHV. © 2012 Elsevier B.V.|
|Appears in Collections:||Scopus 2011-2015|
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