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Title: Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe
Authors: Wansadaj Jaroenram
Narong Arunrut
Wansika Kiatpathomchai
Mahidol University
James Cook University, Australia
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Immunology and Microbiology
Issue Date: 19-Nov-2012
Citation: Journal of Virological Methods. Vol.186, No.1-2 (2012), 36-42
Abstract: Salt-induced self-aggregation of gold nanoparticles (AuNP) carrying unisense ssDNA probes can be prevented specifically by complementary DNA. Loop-mediated isothermal amplification (LAMP) can amplify DNA rapidly. Here, the two techniques were combined to detect yellow head virus (YHV). The LAMP-AuNP method required 60. min for LAMP and 5. min for hybridization of LAMP products to an AuNP-labeled ssDNA probe followed by salt induced probe-particle aggregation to visualize color development. The detection sensitivity of the method was comparable to that of the commercial IQ2000™ nested RT-PCR but only required ∼65. min to produce a result, and did not cross-detect other shrimp viruses. As the LAMP-AuNP protocol only requires a heating block, it offers opportunities for rapid detection of YHV. © 2012 Elsevier B.V.
ISSN: 18790984
Appears in Collections:Scopus 2011-2015

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