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dc.contributor.authorPatumporn Jian-umpunkulen_US
dc.contributor.authorCharin Thepthaien_US
dc.contributor.authorNisachon Apiwaten_US
dc.contributor.authorWarangkana Chantimaen_US
dc.contributor.authorKanokwan Poomputsaen_US
dc.contributor.authorNatpapas Wiriyachaipornen_US
dc.contributor.authorTararaj Dharakulen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherKing Mongkuts University of Technology Thonburien_US
dc.contributor.otherThailand National Science and Technology Development Agencyen_US
dc.identifier.citationJournal of Virological Methods. Vol.185, No.1 (2012), 24-31en_US
dc.description.abstractA new modified triple-antigen detection test was developed for the direct detection of the influenza A virus. The nucleoprotein (NP), matrix (M), and non-structural (NS1) proteins were used as target antigens because they are abundant in infected cells. Monoclonal antibodies specific to the NP, M, and NS1 proteins were generated. The antibody pairs were selected and evaluated for their reactivity individually and in combination in the triple-antigen detection using sandwich ELISA. Triple-antigen detection demonstrated a higher sensitivity than individual antigen detection when tested with both the H1N1 and H3N2 influenza A viruses. This was illustrated by the 4-fold lower limit of detection of the triple-antigen test than the individual antigen detection test. The findings demonstrated that the sensitivity of influenza A antigen detection was improved with the triple-antigen detection system as compared to individual antigen detection. Therefore, this technique could be a useful tool fo r the direct detection of cell-associated influenza A antigen. Furthermore, it could provide a basis for the development of a rapid triple-antigen test for influenza A diagnosis. © 2012 Elsevier B.V.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleImproved sensitivity of influenza A antigen detection using a combined NP, M, and NS1 sandwich ELISAen_US
Appears in Collections:Scopus 2011-2015

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