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|Title:||Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro|
Daniel H. Paris
Stuart D. Blacksell
Thailand National Center for Genetic Engineering and Biotechnology
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.106, No.10 (2012), 632-635|
|Abstract:||The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. © 2012 Royal Society of Tropical Medicine and Hygiene.|
|Appears in Collections:||Scopus 2011-2015|
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