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|Title:||A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos|
|Authors:||Catrin E. Moore|
Stuart D. Blacksell
Richard G. Jarman
Robert V. Gibbons
Sue J. Lee
Paul N. Newton
Armed Forces Research Institute of Medical Sciences, Thailand
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||American Journal of Tropical Medicine and Hygiene. Vol.87, No.1 (2012), 171-178|
|Abstract:||Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4%(95%confidence interval [CI] = 51-78) (Xcyton), 69.2% (95%CI = 55-81) (Panb io), however 96.2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.|
|Appears in Collections:||Scopus 2011-2015|
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