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|Title:||Activation of nuclear factor kappa B in peripheral blood mononuclear cells from malaria patients|
Faculty of Medicine, Thammasat University
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||Malaria Journal. Vol.11, (2012)|
|Abstract:||Background: Malaria parasites and their products can activate a specific immune response by stimulating cytokine production in the hosts immune cells. Transcription nuclear factor kappa B (NF-?B) is an important regulator for the control of many pro-inflammatory genes, such as interleukin-1 (IL-1) and tumor necrosis factor (TNF). The activation and expression of NF-?B p65 in peripheral blood mononuclear cells (PBMCs) of malaria patients were investigated and correlated with the levels of IL-10 and TNF to study the nature of NF-?B p65 and its linkage to inflammatory cytokines. Methods: The sample group comprised 33 patients admitted with malaria caused by Plasmodium vivax (n = 11), uncomplicated Plasmodium falciparum (n = 11), and complicated Plasmodium falciparum (n = 11). Peripheral blood was collected at admission and on day 7 for PBMC isolation. Healthy subjects were used as a control group. The expressions of NF-?B p65 in the PBMCs from malaria patients and the plasma levels of IL-10 and TNF were measured by using enzyme-linked immunosorbent assay (ELISA). The immunofluorescence technique was used to determine NF-?B nuclear translocation. Results: At admission, patients with P. vivax and uncomplicated P. falciparum had significantly elevated phospho- F-?B p65 levels in the PBMCs compared with those of healthy controls. However, patients with complicated P. falciparum malaria had decreased levels of phospho-NF-?B p65. On day 7 post-treatment, significantly increased phospho-NF-?B p65 was found in the PBMCs of patients with complicated P. falciparum, compared with healthy controls. The plasma level of IL-10 was elevated in day 0 in patients with complicated P. falciparum malaria and was found to be negatively correlated with phospho-NF-?B p65 level (r s = ?0.630, p = 0.038). However, there was no correlation between phospho-NF-?B p65 expression and TNF level in patients with complicated P. falciparum malaria. Conclusions: This is the first report demonstrating alterations in NF-?B p65 activity in the PBMCs of malaria patients. The altered lower features of NF-?B p65 in the PBMCs of patients with complicated P. falciparum at admission could be due to a suppressive effect of high IL-10 associated with complicated P. falciparum malaria. © 2012 Punsawad et al.; licensee BioMed Central Ltd.|
|Appears in Collections:||Scopus 2011-2015|
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