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dc.contributor.authorKesinee Chotivanichen_US
dc.contributor.authorMathirut Mungthinen_US
dc.contributor.authorRonnatrai Ruengweerayuthen_US
dc.contributor.authorRachanee Udomsangpetchen_US
dc.contributor.authorArjen M. Dondorpen_US
dc.contributor.authorPratap Singhasivanonen_US
dc.contributor.authorSasithon Pukrittayakameeen_US
dc.contributor.authorNicholas J. Whiteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherPhramongkutklao College of Medicineen_US
dc.contributor.otherMae Sot General Hospitalen_US
dc.contributor.otherUniversity of Oxforden_US
dc.date.accessioned2018-06-11T04:53:29Z-
dc.date.available2018-06-11T04:53:29Z-
dc.date.issued2012-05-29en_US
dc.identifier.citationMalaria Journal. Vol.11, (2012)en_US
dc.identifier.issn14752875en_US
dc.identifier.other2-s2.0-84861439370en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861439370&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/14317-
dc.description.abstractBackground: Lumefantrine and atovaquone are highly lipophilic anti-malarial drugs. As a consequence absorption is increased when the drugs are taken together with a fatty meal, but the free fraction of active drug decreases in the presence of triglyceride-rich plasma lipoproteins. In this study, the consequences of lipidaemia on anti-malarial drug efficacy were assessed in vitro. Methods: Serum was obtained from non-immune volunteers under fasting conditions and after ingestion of a high fat meal and used in standard Plasmodium falciparum in-vitro susceptibility assays. Anti-malarial drugs, including lumefantrine, atovaquone and chloroquine in five-fold dilutions (range 0.05 ng/ml 1 ug/mL) were diluted in culture medium supplemented with fasting or post-prandial 10% donor serum. The in-vitro drug susceptibility of parasite isolates was determined using the 3 H-hypoxanthine uptake inhibition method and expressed as the concentration which gave 50% inhibition of hypoxanthine uptake (IC 50 ). Results: Doubling plasma triglyceride concentrations (from 160 mg/dL to 320 mg/dL), resulted in an approximate doubling of the IC 50 for lumefantrine (191 ng/mL to 465 ng/mL, P < 0.01) and a 20-fold increase in the IC 50 for atovaquone (0.5 ng/mL to 12 ng/ml; P < 0.01). In contrast, susceptibility to the hydrophilic anti-malarial chloroquine did not change in relation to triglyceride content of th e medium. Conclusions: Lipidaemia reduces the anti-malarial activity of lipophilic anti-malarial drugs. This is an important confounder in laboratory in vitro testing and it could have therapeutic relevance. Keywords: Malaria, Anti-malarial drugs, In vitro-susceptibility © 2012 Chotivanich et al.; licensee BioMed Central Ltd.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861439370&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleThe effects of serum lipids on the in vitro activity of lumefantrine and atovaquone against Plasmodium falciparumen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1186/1475-2875-11-177en_US
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