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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/14328
Title: Burkholderia pseudomallei detection in surface water in Southern Laos using Moore's swabs
Authors: Khamsing Vongphayloth
Sayaphet Rattanavong
Catrin E. Moore
Rattanaphone Phetsouvanh
Vanaporn Wuthiekanun
Amphonesavanh Sengdouangphachanh
Phonlavanh Phouminh
Paul N. Newton
Yves Buisson
Institut de la Francophonie pour la Médecine Tropicale
Mahosot Hospital
University of Oxford
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-May-2012
Citation: American Journal of Tropical Medicine and Hygiene. Vol.86, No.5 (2012), 872-877
Abstract: The causal agent of melioidosis, Burkholderia pseudomallei, has been cultured from paddy fields in the Lao PDR. We carried out a pilot study to examine the relationship between bacterial soil contamination and that of nearby surface waters in Saravane Province. Soil sampling was conducted at a depth of 30 cm (100 holes in a 45 x 45 m grid) at two sites, East and West Saravane. Moore's swabs were used for water sampling of paddy fields, lakes, rivers, boreholes, and storage tanks within 2 km of the two soil sampling sites. B. pseudomallei from soil and water were cultured on Ashdown's agar. Thirty-six percent and 6% of water samples collected around East and West Saravane, respectively, were culture positive for B. pseudomallei. Low pH and high turbidity were independently associated with culture of B. pseudomallei. Most positive water samples were from the Sedone River, downstream of the East Saravane site. Moore's swabs are simple and inexpensive tools for detecting B. pseudomallei in surface waters. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861820216&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/14328
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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