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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/14329
Title: Susceptibility of inbred mice to Rickettsia parkeri
Authors: Britton J. Grasperge
Kathryn E. Reif
Timothy D. Morgan
Piyanate Sunyakumthorn
Joseph Bynog
Christopher D. Paddock
Kevin R. Macaluso
LSU School of Veterinary Medicine
Washington State University Pullman
Mississippi State University
Mahidol University
Centers for Disease Control and Prevention
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-May-2012
Citation: Infection and Immunity. Vol.80, No.5 (2012), 1846-1852
Abstract: Rickettsia parkeri, a member of the spotted fever group Rickettsia, is the causative agent of American boutonneuse fever in humans. Despite the increased recognition of human cases, limited information is available regarding the infection of invertebrate and vertebrate hosts for this emerging tick-borne disease. Toward the development of a viable transmission model and to further characterize the pathology associated with R. parkeri infection, inbred mouse strains (A/J, BALB/c, C3H/HeJ, and C3H/HeN) were intravenously and intradermally inoculated with 10 5 low-passage-number R. parkeri (Portsmouth strain), and infection, gross pathology, and histopathology were scored. Additionally, a quantitative real-time PCR (qPCR) was performed to estimate rickettsial load in heart, lung, spleen, and liver tissues of infected mice at 19 days postinoculation. Of the A/J, BALB/c, and C3H/ HeN mice, none displayed universal pathology consistent with sustained infection. Compared to age-matched control mice, the intravenously inoculated C3H/HeJ mice exhibited marked facial edema and marked splenomegaly upon gross examination, while the intradermally inoculated mice developed characteristic eschar-like lesions. The C3H/HeJ mice also exhibited the greatest concentrations of rickettsial DNA from heart, lung, liver, and spleen samples when examined by qPCR. The similarity of the pathology of human disease and sustained infection suggests that the C3H/HeJ strain of mice is a promising candidate for subsequent experiments to examine the tick transmission, dissemination, and pathology of R. parkeri rickettsiosis. © 2012, American Society for Microbiology.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84861123808&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/14329
ISSN: 10985522
00199567
Appears in Collections:Scopus 2011-2015

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