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Title: Accuracy of a commercial IgM ELISA for the diagnosis of human leptospirosis in Thailand
Authors: Varunee Desakorn
Vanaporn Wuthiekanun
Vipa Thanachartwet
Duangjai Sahassananda
Wirongrong Chierakul
Apichat Apiwattanaporn
Nicholas P. Day
Direk Limmathurotsakul
Sharon J. Peacock
Mahidol University
Udon Thani Rajabhat University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Mar-2012
Citation: American Journal of Tropical Medicine and Hygiene. Vol.86, No.3 (2012), 524-527
Abstract: The Leptospira immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA) has been recommended for the rapid diagnosis of leptospirosis in endemic areas. We conducted a retrospective case-control study of 218 patients (109 leptospirosis cases confirmed by Leptospira culture and/or microscopic agglutination test and 109 control patients with acute febrile illness) to evaluate the diagnostic accuracy of a commercial IgM ELISA (Panbio) in northeast Thailand. Paired serum samples taken on admission and at least 10 days after the onset of symptoms were tested. Using the cutoff value recommended by the manufacturer (11 Panbio units), sensitivity and specificity of IgM ELISA on paired sera were 90.8% and 55.1%. A receiver operating characteristic curve was used to determine the optimal cutoff value. This was 20 Panbio units, which gave a sensitivity and specificity of 76.1% and 82.6%, respectively, on paired sera. We conclude that using either cutoff value, the accuracy of IgM ELISA is limited in our setting. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2011-2015

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