Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorS. Tapaneeyakornen_US
dc.contributor.authorN. Apiwaten_US
dc.contributor.authorW. Maneeprakornen_US
dc.contributor.authorS. Chumsengen_US
dc.contributor.authorC. Thepthaien_US
dc.contributor.authorT. Dharakulen_US
dc.contributor.otherThailand National Nanotechnology Centeren_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationTechnical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012. (2012), 206-209en_US
dc.description.abstractImmunodiagnosis is an attractive choice for quick and reliable cancer screening. p16 INK4A (p16) is a promising biomarkers for cervical cancer. The over-expression of p16 is linked to the oncogenic transformation. Here, monoclonal antibodies (mAbs) specific to p16 were produced by using hybridoma technology for immunodiagnostic purposes. The p16 was expressed in E.coli and purified by affinity chromatography. The protein was used for mouse immunization. Hybridomas producing antibodies to p16 were selected by ELISA. The mAbs were purified by protein A and G columns. The specificity and sensitivity of the antibodies were determined by Western blotting and ELISA. The selected anti-p16 mAb with highest sensitivity was conjugated to gold nanoparticles (AuNPs) by non-covalent adsorption. Immunochromatographic (IC) tests by using conjugated anti-p16 mAb and purified p16 were successfully performed for the first time. This method might be further used for early detection of cervical cancer.en_US
dc.rightsMahidol Universityen_US
dc.subjectMaterials Scienceen_US
dc.titleImmunodiagnosis for cervical cancer using antibody-gold nanoparticle conjugateen_US
dc.typeConference Paperen_US
Appears in Collections:Scopus 2011-2015

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.