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Title: Role of CD61<sup>+</sup>cells in thrombocytopenia of dengue patients
Authors: Sansane Noisakran
Nattawa Onlamoon
Kovit Pattanapanyasat
Hui Mien Hsiao
Puchare Songprakhon
Nasikar Angkasekwinai
Kulkany Chokephaibulkit
Francoi Villinger
Aftab A. Ansari
Guey Chuen Perng
Emory University School of Medicine
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Emory University
National Cheng Kung University
Keywords: Medicine
Issue Date: 1-Nov-2012
Citation: International Journal of Hematology. Vol.96, No.5 (2012), 600-610
Abstract: Although hematological disorders with salient features of thrombocytopenia have been well documented in dengue patients, the role of CD61-expressing platelets and the megakaryocytic cell lineage in the pathogenesis of dengue virus (DENV) infection remains largely unexplored. A prospective observational study was performed using blood samples andPBMCsfromdengue-confirmed patients, as well as from rhesus monkeys (RM) experimentally infected with DENV. Immunohistochemical staining and FACS techniques were applied to evaluate the frequencies of CD61 + cells that contained DENV antigen. Highly enriched population of CD61 + cells was also isolated from acute DENV-infected RM and assayed for DENV RNA by quantitative RT-PCR. Results revealed that DENV antigen was found in small vesicles of varying size, and more frequently in anucleated cells associated with platelets in dengue patients. The DENV antigen-containing cells were CD61 + And appeared to share characteristics of egakaryocytes. Kinetic profiles of CD61 + cells from DENV-infected RM revealed a transient increase in CD61 + CD62P + cells early after DENV infection. DENV RNA in a highly enriched population of CD61 + cells fromthe infectedRMwas observed during acute stage. Our results indicate that virus containing CD61 + Cells may be directly linked to the platelet dysfunction and low platelet count characteristics of dengue patients. © 2012 The Japanese Society of Hematology.
ISSN: 18653774
Appears in Collections:Scopus 2011-2015

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