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dc.contributor.authorLorena Diazen_US
dc.contributor.authorPattarachai Kiratisinen_US
dc.contributor.authorRodrigo E. Mendesen_US
dc.contributor.authorDiana Panessoen_US
dc.contributor.authorKavindra V. Singhen_US
dc.contributor.authorCesar A. Ariasen_US
dc.contributor.otherUniversity of Texas Medical School at Houstonen_US
dc.contributor.otherUniversidad El Bosqueen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherJMI Laboratories Incorporateden_US
dc.identifier.citationAntimicrobial Agents and Chemotherapy. Vol.56, No.7 (2012), 3917-3922en_US
dc.description.abstractNonmutational resistance to linezolid is due to the presence of cfr, which encodes a methyltransferase responsible for methylation of A2503 in the 23S rRNA. The cfr gene was first described in animal isolates of staphylococci, and more recently, it has been identified in Staphylococcus aureus from human clinical infections, including in an outbreak of methicillin-resistant S. aureus. In enterococci, cfr has been described in an animal isolate of Enterococcus faecalis from China. Here, we report an isolate of linezolid-resistant E. faecalis (603-50427X) recovered from a patient in Thailand who received prolonged therapy with the antibiotic for the treatment of atypical mycobacterial disease. The isolate lacked mutations in the genes coding for 23S rRNA and L3 and L4 ribosomal proteins and belonged to the multilocus sequence type (MLST) 16 (ST16), which is commonly found in enterococcal isolates from animal sources. Resistance to linezolid was associated with the presence of cfr on an ∼97-kb transferable plasmid. The cfr gene environment exhibited DNA sequences similar to those of other cfr-carrying plasmids previously identified in staphylococci (nucleotide identity, 99 to 100%). The cfr-carrying plasmid was transferable by conjugation to a laboratory strain of E. faecalis (OG1RF) but not to Enterococcus faecium or S. aureus. The cfr gene was flanked by IS256-like sequences both upstream and downstream. This is the first characterization of the potential horizontal transferability of the cfr gene from a human linezolid-resistant isolate of E. faecalis. Copyright © 2012, American Society for Microbiology. All Rights Reserved.en_US
dc.rightsMahidol Universityen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleTransferable plasmid-mediated resistance to linezolid due to cfr in a human clinical isolate of Enterococcus faecalisen_US
Appears in Collections:Scopus 2011-2015

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