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Title: Cloning and expression of 130-kd mosquito-larvicidal δ-endotoxin gene of Bacillus thuringiensis var. Israelensis in Escherichia coli
Authors: Chanan Angsuthanasombat
Wipa Chungjatupornchai
Sunee Kertbundit
Plernpis Luxananil
Chatri Settasatian
Prapon Wilairat
Sakol Panyim
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jul-1987
Citation: MGG Molecular & General Genetics. Vol.208, No.3 (1987), 384-389
Abstract: Five recombinant E. coli clones exhibiting toxicity to Aedes aegypti larvae were obtained from a library of 800 clones containing XbaI DNA fragments of 110 kb plasmid from B. thuringiensis var. israelensis. All the five clones (pMU 14/258/303/388/679) had the same 3.8-kb insert and encoded a major protein of 130 kDa which was highly toxic to A. aegypti larvae. Three clones (pMU 258/303/388) transcribed the 130 kD a gene in the same direction as that of lac Z promoter of pUC12 vector whereas the transcription of the other two (pMU 14/679) was in the opposite direction. A 1.9-kb fragment of the 3.8 kb insert coded for a protein of 65 kDa. Partial DNA sequence of the 3.8 kb insert, corresponding to the 5′-terminal of the 130 kDa gene, revealed a continuous reading frame, a Shine-Dalgarno sequence and a tentative 5′-regulatory region. These results demonstrated that the 3.8 kb insert is a minimal DNA fragment containing a regulatory region plus the coding sequence of the 130 kDa protein that is highly toxic to mosquito larvae. © 1987 Springer-Verlag.
ISSN: 16174623
Appears in Collections:Scopus 1969-1990

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