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Title: Detection of β-thalassemia and hemoglobin E genes in Thai by a DNA amplification technique
Authors: Pranee Winichagoon
Jiraporn Kownkon
Pathai Yenchitsomanus
Varaporn Thonglairoam
Nopadol Siritanaratkul
Suthat Fucharoen
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Jul-1989
Citation: Human Genetics. Vol.82, No.4 (1989), 389-390
Abstract: Enzymatic DNA amplification and polyacrylamide gel electrophoresis, which demonstrate different sizes of DNA fragments, were used to detect the common mutations causing β-thalassemia and hemoglobin (Hb) E in Thai people. The 4-bp deletion at codons 41 and 42 can be detected directly by polyacrylamide gel electrophoresis and ethidium bromide staining. Whereas the nonsense mutations at codon 17 (AAG →TAG) and Hb E (GAG→AAG at codon 26) were detected after digestion of the amplified DNA with the enzymes MaeI and MnlI, respectively. © 1989 Springer-Verlag.
ISSN: 14321203
Appears in Collections:Scopus 1969-1990

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