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Title: Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.
Authors: V. Boonsaeng
K. Chansiri
P. Vilasineekul
P. Wilairat
S. Panyim
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-1989
Citation: The Southeast Asian journal of tropical medicine and public health. Vol.20, No.4 (1989), 519-522
Abstract: A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
ISSN: 01251562
Appears in Collections:Scopus 1969-1990

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