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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/15971
Title: Diagnosis of Plasmodium vivax malaria using a specific deoxyribonucleic acid probe
Authors: Wendy A. Relf
Robyn E. Boreham
Pramuan Tapchaisri
Srisin Khusmith
Andrew Healey
Peter Upcroft
Savanat Tharavanij
Chev Kidson
Queensland Institute of Medical Research
Menzies School of Health Research
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-1990
Citation: Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.84, No.5 (1990), 630-634
Abstract: A deoxyribonucleic acid (DNA) probe which specifically distinguishes Plasmodium vivax from P. falciparum malaria has been derived from a P. vivax genomic DNA library. This probe, VPL101, consists of 3A 2 kilobase pairs and does not hybridize with up to 6 Ig of human or P. falciparum DNA. VPL101 contains at least two copies of a 205 base pair repeat sequence. The subcloned repeat probe, VPL101 5, reacted with 73 of 76 microscopically diagnosed P. vivax samples but not with any of 17 human DNA samples or any of 8 P. falciparum DNA samples from cultured parasites. It was possible to detect P. vivax in mixed infections in which only P. falciparum parasites were identifiable by microscopy. This P. vivax DNA probe provides a useful epidemiological tool for malaria control programmes. © 1990, Royal Society of Tropical Medicine and Hygiene. All Rights Reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025077615&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/15971
ISSN: 18783503
00359203
Appears in Collections:Scopus 1969-1990

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