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Title: Simultaneous measurement of quinine and quinidine in human plasma, whole blood, and erythrocytes by high-performance liquid chromatography with fluorescence detection
Authors: Michael D. Edstein
Aree Prasitthipayong
Arunee Sabchareon
Tan Chongsuphajaisiddhi
H. Kyle Webster
Mahidol University
Keywords: Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-1990
Citation: Therapeutic Drug Monitoring. Vol.12, No.5 (1990), 493-500
Abstract: A high-performance liquid chromatographic method with fluorescence detection is described for the simultaneous measurement of quinine and quinidine in plasma, whole blood, and erythrocytes. The compounds were separated on an Ultrasphere C18 reversed-phase column (25 cm x 4.6 mm inside diameter, 5 μm particle size) using a mobile phase of acetonitrile/water/triethylamine (11:88:1, vol/vol) at pH 2.5. The method, simple, accurate, and selective, requires only a single-step liquid-liquid extraction and uses the structurally similar alkaloid, cinchonine, as the internal standard. The commercial impurities, dihydroquinine and dihydroquinidine, and unknown metabolites were well resolved from the parent drugs. The assay is precise, with interassay coefficients of variation 7.0% and an accuracy of 7.3% over a concentration range of 0.125 to 4.0 μg/0.25 ml. The extraction recoveries of the two drugs were similar, averaging 82.9% for quinine and 79.3% for quinidine from the three biological fluids. The clinical application of the method for routine drug monitoring and for estimating the pharmacokinetics of quinine and quinidine in man are discussed. © 1990 Raven Press, Ltd., New York.
ISSN: 15363694
Appears in Collections:Scopus 1969-1990

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