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Title: Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in Thailand
Authors: Tirasak Pasharawipas
Surasak Thaikua
Siriporn Sriurairatana
Lila Ruangpan
Sataporn Direkbusarakum
Jaroon Manopvisetcharean
Timothy W. Flegel
Mahidol University
Rangsit University
Chanthaburi Coastal Fisheries Research and Development Center
Walailak University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Dec-2005
Citation: Virus Research. Vol.114, No.1-2 (2005), 63-69
Abstract: A bacteriophage was isolated together with Vibrio harveyi (VH) 1114 a from a black tiger shrimp-rearing pond in Thailand. By negative staining transmission electron microscopy (TEM), the phage had an icosahedral head (diameter 60-62 nm), a rigid, non-contractile tail (9-10 nm x 100-120 nm) without a collar or terminal fibers and a genome of double stranded DNA of approximately 80 kb as determined by analysis of restriction enzyme digestion fragments. Since these features would place it in the family Siphoviridae, it was tentatively named V. harveyi siphoviridae-like phage or VHS1. VHS1 could also infect two VH reference strains LMD 22.30 and LMD 80.33 (=ATCC 14126) but yielded smaller plaques than with VH1114. The phage tolerated temperatures as high as 60°C for up to 2 h and overnight exposure to a broad range of pH. VHS1 lysogens of VH1114 were unstable, contained unaltered VHS1 DNA, were immune to VHS1 lysis and spontaneously released VHS1 in liquid cultures. Approximately 20 kb of the genome has been sequenced and deposited at GenBank but it mostly showed no significant homology with existing sequences in the database. © 2005 Elsevier B.V. All rights reserved.
ISSN: 01681702
Appears in Collections:Scopus 2001-2005

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