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Title: Evaluation of a new single-parameter volumetric flow cytometer (CyFlow <sup>green</sup>) for enumeration of absolute CD4<sup>+</sup> T lymphocytes in human immunodeficiency virus type 1-infected Thai patients
Authors: Kovit Pattanapanyasat
Surada Lerdwana
Egarit Noulsri
Thanyanan Chaowanachan
Punneeporn Wasinrapee
Natthaga Sakulploy
Vallerut Pobkeeree
Orapin Suksripanich
Sombat Thanprasertsuk
Thomas J. Spira
Jordan W. Tappero
William C. Levine
Mahidol University
Thailand Ministry of Public Health
National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention
Centers for Disease Control and Prevention
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Dec-2005
Citation: Clinical and Diagnostic Laboratory Immunology. Vol.12, No.12 (2005), 1416-1424
Abstract: Use of the standard dual-platform How cytometric method for determination of CD4+ T-lymphocyte counts, which needs both a How cytometer (FCM) and hematological analyzer, would inevitably lead to increased variability. The development of new single-platform (SP) FCMs that provide direct CD4+ T-lymphocyte counts for improved assay precision and accuracy have recently attracted attention. This study evaluated one of those systems, CyFlow green (Partec), a single-parameter SP volumetric FCM. The performance of CyFlowgreen was compared with those of two reference standard SP microbead-based technologies of the three-color TruCOUNT tube with the FACScan FCM and a two-color FACSCount system (Becton Dickinson Biosciences). Absolute CD4+ and CD8+ T-lymphocyte counts in 200 human immunodeficiency virus type 1-seropositive blood specimens were determined. Statistical analysis for correlation and agreement were performed. A high correlation of absolute CD4 counts was shown when those obtained with CyFlowgreen were compared with those obtained with the bead-based three-color TruCOUNT system (R2 = 0.96; mean bias, -69.1 cells/μl; 95% confidence interval [CI], -225.7 to +87.5 cells/μl) and the FACSCount system (R2 = 0.97; mean bias, -40.0 cells/μl; 95% CI, -165.1 to +85.1 cells/μl). The correlation of the CD4+ T-lymphocyte counts obtained by the two bead-based systems was high (R2 = 0.98). Interestingly, CyFlowgreen yielded CD4+ T-lymphocyte counts that were 21.8 and 7.2 cells/μl lower than those obtained with the TruCOUNT and the FACSCount systems, respectively, when CD4+ T-lymphocyte counts were <250 CD4+ T-lymphocyte counts/μl range or 17.3 and 5.8 cells/μl less, respectively, when CD4+ T-lymphocyte counts were <200 cells/μl. The single-parameter CyFlow green volumetric technology performed well in comparison with the performance of the standard SP bead-based FCM system. However, a multicenter comparative study is needed before this FCM machine is implemented in resource-limited settings. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
ISSN: 1071412X
Appears in Collections:Scopus 2001-2005

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