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Title: The Burkholderia pseudomallei RpoE (AlgU) operon is involved in environmental stress tolerance and biofilm formation
Authors: Sunee Korbsrisate
Muthita Vanaporn
Phansupa Kerdsuk
Wannapa Kespichayawattana
Paiboon Vattanaviboon
Pornpimon Kiatpapan
Ganjana Lertmemongkolchai
Mahidol University
Chulabhorn Research Institute
Rangsit University
Khon Kaen University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 15-Nov-2005
Citation: FEMS Microbiology Letters. Vol.252, No.2 (2005), 243-249
Abstract: Burkholderia pseudomallei, the causative agent of melioidosis, can be isolated from soil and water. To persist, adapt and survive within and outside their human host, bacteria rely on regulatory mechanisms that allow them to respond rapidly to stressful situations. We have examined the possible role of B. pseudomallei alternative sigma factor σE(RpoE) in the stress response and found that rpoE and its putative regulators (bprE-rseB-mucD) are transcribed in a single transcriptional unit. Inactivation of the rpoE operon changed the B. pseudomallei phenotype. Changes included increased susceptibility to killing by menadione and H2O2, susceptibility to high osmolarity, reduced ability to form biofilms, and reduced survival in macrophage J774A.1. Therefore, we conclude that rpoE controls gene expression that contributes, at least in part, to B. pseudomallei adaptation to adverse environmental conditions. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 03781097
Appears in Collections:Scopus 2001-2005

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