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Title: Proteomic profiling of cholangiocarcinoma cell line treated with pomiferin from Derris malaccensis
Authors: Jisnuson Svasti
Chantragan Srisomsap
Pantipa Subhasitanont
Siriporn Keeratichamroen
Daranee Chokchaichamnankit
Lukana Ngiwsara
Nitirat Chimnoi
Somchai Pisutjaroenpong
Supanna Techasakul
Shui Tein Chen
Chulabhorn Research Institute
Mahidol University
Kasetsart University
Academia Sinica Taiwan
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Nov-2005
Citation: Proteomics. Vol.5, No.17 (2005), 4504-4509
Abstract: Pomiferin, a prenylated isoflavonoid from Derris malaccensis with strong anti-fungal and anti-oxidant activities, showed cytotoxic activity towards human cholangiocarcinoma cells (HuCCA-1), with IC50 of 0.9 μg/mL. Pomiferin caused apoptosis, detectable by DNA fragmentation. Two-dimensional PAGE showed increased expression of 12 proteins, namely glucose-regulated protein 75 (grp 75), calcyclin (S100A6), degraded cytokeratin 19, ATP synthase D, ribosomal protein P0, degraded cytokeratin 18 (two spots pI/MW 6.03/29.9 and pI/MW 4.66/21.5), cofilin, annexin A1, triose phosphate isomerase, peroxiredoxin-1, calgizzarin, and profilin. In contrast, cytokeratins (CK) 7, 18 and 19 were down-regulated, and were shown by 1-DE immunodetection to be degraded. © 2005 WILEY-VCH Verlag GmbH & Co. KGaA.
ISSN: 16159853
Appears in Collections:Scopus 2001-2005

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