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Title: Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae
Authors: Boonhiang Promdonkoy
Patcharee Promdonkoy
Sakol Panyim
Thailand National Center for Genetic Engineering and Biotechnology
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Nov-2005
Citation: FEMS Microbiology Letters. Vol.252, No.1 (2005), 121-126
Abstract: Cry4Ba is a δ-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic δ-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC50= 140 ng ml-1) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC50values of 350 and 250 ng ml-1, respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti andC. quinquefasciatus larvae (LC50= 7 and 20 ng ml-1, respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 03781097
Appears in Collections:Scopus 2001-2005

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