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dc.contributor.authorHongfang Qiuen_US
dc.contributor.authorCuihua Huen_US
dc.contributor.authorFan Zhangen_US
dc.contributor.authorJiunn Hwang Gwoen_US
dc.contributor.authorMark J. Swansonen_US
dc.contributor.authorCheunchit Boonchirden_US
dc.contributor.authorAlan G. Hinnebuschen_US
dc.contributor.otherNational Institute of Child Health and Human Developmenten_US
dc.contributor.otherNational Institutes of Health, Bethesdaen_US
dc.contributor.otherNanchang Universityen_US
dc.contributor.otherLouisiana Tech Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationMolecular and Cellular Biology. Vol.25, No.9 (2005), 3461-3474en_US
dc.description.abstractTranscriptional activation by Gcn4p is enhanced by the coactivators SWI/SNF, SAGA, and Srb mediator, which stimulate recruitment of TATA binding protein (TBP) and polymerase II to target promoters. We show that wild-type recruitment of SAGA by Gcn4p is dependent on mediator but independent of SWI/SNF function at three different promoters. Recruitment of mediator is also independent of SWI/SNF but is enhanced by SAGA at a subset of Gcn4p target genes. Recruitment of all three coactivators to ARG1 is independent of the TATA element and preinitiation complex formation, whereas efficient recruitment of the general transcription factors requires the TATA box. We propose an activation pathway involving interdependent recruitment of SAGA and Srb mediator to the upstream activation sequence, enabling SWI/SNF recruitment and the binding of TBP and other general factors to the promoter. We also found that high-level recruitment of Tra1p and other SAGA subunits is independent of the Ada2p/Ada3p/Gcn5p histone acetyltransferase module but requires Spt3p in addition to subunits required for SAGA integrity. Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleInterdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4pen_US
Appears in Collections:Scopus 2001-2005

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