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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/16352
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dc.contributor.authorKovit Pattanapanyasaten_US
dc.contributor.authorHla Shainen_US
dc.contributor.authorEgarit Noulsrien_US
dc.contributor.authorSurada Lerdwanaen_US
dc.contributor.authorCharin Thepthaien_US
dc.contributor.authorVaripin Prasertsilpaen_US
dc.contributor.authorSirirat Likanonsakulen_US
dc.contributor.authorPrakit Yothipitaken_US
dc.contributor.authorSomboon Nookhaien_US
dc.contributor.authorAchara Eksaengsrien_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherThailand Government Pharmaceutical Organizationen_US
dc.contributor.otherBamrasnaradura Hospitalen_US
dc.contributor.otherChonburi Regional Hospitalen_US
dc.contributor.otherThai Red Cross Agencyen_US
dc.date.accessioned2018-06-21T08:09:36Z-
dc.date.available2018-06-21T08:09:36Z-
dc.date.issued2005-05-01en_US
dc.identifier.citationCytometry Part B - Clinical Cytometry. Vol.65, No.1 (2005), 29-36en_US
dc.identifier.issn15524949en_US
dc.identifier.other2-s2.0-18144416171en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=18144416171&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/16352-
dc.description.abstractBackground: The current method of CD4 enumeration in Thailand, based on the three-tube, three-color method recommended by the Centers for Disease Control and Prevention, is expensive and thus unavailable to most patients who have the human immunodeficiency virus (HIV). Less expensive, simpler protocols (i.e., PanLeucogating and primary CD4 gating) have been described but require more published validation data to gain widespread acceptance. We describe a multicenter evaluation of the PanLeucogating method. Methods: The PanLeucogating method using generic reagents was evaluated in comparison with the standard three-tube, three-color method using commercial reagents. Percentage of CD4 + T cells among lymphocytes and absolute CD4+ T-cell counts were determined in 611 HIV-infected individuals recruited from four sites. Linear regression and Bland-Altman tests were used for statistical analysis. Results: The correlation of percentage of CD4+ T cells and absolute CD4+ T-cell counts obtained with the PanLeucogating strategy and the standard predicate method was high (r2 = 0.96 and 0.95, respectively, for the entire study population and r2 > 0.95 and 0.93, respectively, for each study group). Absolute CD4+ T-cell counts of the overall study pool and of the two subdivisions of absolute CD4+ T-cell counts (i.e., 0-250 cells/μl and >250 cells/μl) derived from the two methods demonstrated excellent agreement, with mean biases of +18 cells/μl, +11 cells/μl, and +24 cells/μl, respectively. Conclusions: These observations demonstrate that CD4 enumeration by PanLeucogating is reliable and can be performed to an identical standard in a quality-assured network of collaborating laboratories as a new cost-effective approach to HIV monitoring. © 2005 Wiley-Liss, Inc.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=18144416171&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMedicineen_US
dc.titleA multicenter evaluation of the PanLeucogating method and the use of generic monoclonal antibody reagents for CD4 enumeration in HIV-infected patients in Thailanden_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1002/cyto.b.20052en_US
Appears in Collections:Scopus 2001-2005

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