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dc.contributor.authorKunchit Judprasongen_US
dc.contributor.authorMathuros Ornthaien_US
dc.contributor.authorAtitaya Siripinyanonden_US
dc.contributor.authorJuwadee Shiowatanaen_US
dc.contributor.otherMahidol Universityen_US
dc.identifier.citationJournal of Analytical Atomic Spectrometry. Vol.20, No.11 (2005), 1191-1196en_US
dc.description.abstractA continuous-flow dialysis (CFD) method with on-line inductively coupled plasma optical emission spectrometric (ICP-OES) simultaneous multielement measurement for the study of in vitro mineral bioavailability was developed. The method was based on a simulated gastric digestion in a batch system followed by a continuous-flow intestinal digestion. The simulated intestinal digestion was performed in a dialysis bag placed inside a channel in a flowing stream of dialyzing solution (NaHCO3). The mineral concentrations in the dialysate were determined by ICP-OES using Y and Sc as internal standards. The pH of the dialysate was also monitored on-line to ensure pH changes similar to the situation in the gastrointestinal tract. The developed system was applied to determining the dialysability of five essential elements (Ca, Mg, P, Fe, Zn) for various kinds of foods, i.e., milk-based infant formula reference material (NIST SRM 1846), milk powder, kale, mungbean, chicken meat, jasmine rice, and Acacia pennata. The dialysis profiles of elements and pH change profiles can be useful in understanding the dialysis change and factors affecting dialysability. All studied elements were rapidly dialysed in the first 30 min of simulated intestinal digestion. It is expected that this system will be useful for estimation of dialysability and for studying the mutual effects of components in food. © The Royal Society of Chemistry 2005.en_US
dc.rightsMahidol Universityen_US
dc.titleA continuous-flow dialysis system with inductively coupled plasma optical emission spectrometry for in vitro estimation of bioavailabilityen_US
Appears in Collections:Scopus 2001-2005

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