Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/16544
Title: Nasal immunization with a malaria transmission-blocking vaccine candidate, Pfs25, induces complete protective immunity in mice against field isolates of Plasmodium falciparum
Authors: Takeshi Arakawa
Ai Komesu
Hitoshi Otsuki
Jetsumon Sattabongkot
Rachanee Udomsangpetch
Yasunobu Matsumoto
Naotoshi Tsuji
Yimin Wu
Motomi Torii
Takafumi Tsuboi
University of the Ryukyus
Ehime University, School of Medicine
Armed Forces Research Institute of Medical Sciences, Thailand
Mahidol University
Graduate School of Agricultural and Life Sciences The University of Tokyo
Central Region Agricultural Research Center NARO
National Institute of Allergy and Infectious Diseases
Ehime University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Nov-2005
Citation: Infection and Immunity. Vol.73, No.11 (2005), 7375-7380
Abstract: Malaria transmission-blocking vaccines based on antigens expressed in sexual stages of the parasites are considered one promising strategy for malaria control. To investigate the feasibility of developing noninvasive mucosal transmission-blocking vaccines against Plasmodium falciparum, intranasal immunization experiments with Pichia pastoris-expressed recombinant Pfs25 proteins were conducted. Mice intranasally immunized with the Pfs25 proteins in the presence of a potent mucosal adjuvant cholera toxin induced robust systemic as well as mucosal antibodies. All mouse immunoglobulin G (IgG) subclasses except IgG3 were found in serum at comparable levels, suggesting that the immunization induced mixed Th1 and Th2 responses. Consistent with the expression patterns of the Pfs25 proteins in the parasites, the induced immune sera specifically recognized ookinetes but not gametocytes. In addition, the immune sera recognized Pfs25 proteins with the native conformation but not the denatured forms, indicating that mucosal immunization induced biologically active antibodies capable of recognizing conformational epitopes of native Pfs25 proteins. Feeding Anopheles dirus mosquitoes with a mixture of the mouse immune sera and gametocytemic blood derived from patients infected with P. falciparum resulted in complete interference with oocyst development in mosquito midguts. The observed transmission-blocking activities were strongly correlated with specific serum antibody titers. Our results demonstrated for the first time that a P. falciparum transmission-blocking vaccine candidate is effective against field-isolated parasites and may justify the investigation of noninvasive mucosal vaccination regimens for control of malaria, a prototypical mucosa-unrelated mosquito-borne parasitic disease. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=27744520417&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/16544
ISSN: 00199567
Appears in Collections:Scopus 2001-2005

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