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Title: Short report: Detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction
Authors: Tasawan Singhsilarak
Wattana Leowattana
Sornchailoo Areesuwan
Varee Wongchotigul
Ju Jiang
Allen L. Richards
George Watt
Mahidol University
Naval Medical Research Center
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-May-2005
Citation: American Journal of Tropical Medicine and Hygiene. Vol.72, No.5 (2005), 640-641
Abstract: Orientia tsutsugamushi infection causes scrub typhus, a common zoonosis of rural Asia. Orientia tsutsugamushi was recently detected by a real-time quantitative polymerase chain reaction (qPCR) assay in animal specimens. We evaluated the same qPCR assay in specimens obtained from patients with serologically proven scrub typhus infections. The 47-kDa qPCR assay was more sensitive than was mouse inoculation; it was reactive in whole blood specimens from all 10 isolate-positive patients and in 7 of 17 isolate-negative individuals (P = 0.003, Fisher's two-tailed exact test). As few as 1,076 O. tsutsugamushi copies/μL were detected in whole blood. Four of 7 sera from isolate-proven scrub typhus infections were also reactive by qPCR. The assay was unreactive in all 12 individuals without scrub typhus infection. This is the first demonstration of a sensitive and specific real-time qPCR assay for human scrub typhus infection. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene.
ISSN: 00029637
Appears in Collections:Scopus 2001-2005

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