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Title: Analysis of sequence variation in Gnathostoma spinigerum mitochondrial DNA by single-strand conformation polymorphism analysis and DNA sequence
Authors: Charinthon Ngarmamonpirat
Jitra Waikagul
Songsak Petmitr
Paron Dekumyoy
Wichit Rojekittikhun
Malinee T. Anantapruti
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2005
Citation: Parasitology International. Vol.54, No.1 (2005), 65-68
Abstract: Morphological variations were observed in the advance third stage larvae of Gnathostoma spinigerum collected from swamp eel (Fluta alba), the second intermediate host. Larvae with typical and three atypical types were chosen for partial cytochrome c oxidase subunit I (COI) gene sequence analysis. A 450 bp polymerase chain reaction product of the COI gene was amplified from mitochondrial DNA. The variations were analyzed by single-strand conformation polymorphism and DNA sequencing. The nucleotide variations of the COI gene in the four types of larvae indicated the presence of an intra-specific variation of mitochondrial DNA in the G. spinigerum population. © 2005 Elsevier Ireland Ltd. All rights reserved.
ISSN: 13835769
Appears in Collections:Scopus 2001-2005

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