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Title: Evaluation of polymerase chain reaction and restriction enzyme analysis for routine identification of mycobacteria: Accuracy, rapidity, and cost analysis
Authors: Therdsak Prammananan
Wattana Cheunoy
Preeyawit Na-Ubol
Nipa Tingtoy
Somboon Srimuang
Angkana Chaiprasert
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Keywords: Medicine
Issue Date: 1-Sep-2005
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.5 (2005), 1252-1260
Abstract: Polymerase chain reaction and restriction enzyme analysis (PCR-REA) of the hsp65 gene was evaluated for use as a routine identification method for identifying mycobacteria. The accuracy, rapidity, and cost were assessed compared with the conventional biochemical method. Five hundred and forty-one mycobacterial clinical isolates obtained from the Department of Microbiology, Faculty of Medicine at Siriraj Hospital, Mahidol University, were submitted for PCR-REA and biochemical identification. PCR-REA showed high concordant result with 100, 96.2, and 94.1% for identification of Mycobacterium tuberculosis, rapid- and slow-growing mycobacteria, respectively. Discordant results were obtained from 24 (4.4%) out of 541 isolates, consisting of 9 rapid growers (6 M. chelonae, 2 M. abscessus, and 1 M. fortuitum) and 15 slow growers (9 M. scrofulaceum, 2 M. gordonae, 1 M. avium, 1 M. kansasii, 1 M. malmoense, and 1 M. terrae complex). PCR-REA demonstrated not only accurate results but was also less expensive (2.1 US$/sample). This method was rapid with a turn-around time of 30 hours compared with 2-4 weeks for the conventional method.
ISSN: 01251562
Appears in Collections:Scopus 2001-2005

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