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Title: Bottle and biochemical assays on temephos resistance in Aedes aegypti in Thailand
Authors: Visut Saelim
William G. Brogdon
Jirasak Rojanapremsuk
Saravudh Suvannadabba
Wongdyan Pandii
James W. Jones
Ratana Sithiprasasna
Pattani Provincial Public Health Office
National Center for Infectious Diseases
Mahidol University
Thailand Ministry of Public Health
Armed Forces Research Institute of Medical Sciences, Thailand
Keywords: Medicine
Issue Date: 1-Mar-2005
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.36, No.2 (2005), 417-425
Abstract: The bottle bioassay measuring the time-mortality rate is a simplified procedure for detecting insecticide resistance. It can be used with a biochemical microplate assay to identify the mechanism involved. This integrated approach was used to detect temephos resistance in Aedes aegypti from Nonthaburi (lowest use) and Roi Et (highest use). Ae. aegypti BKK1 laboratory strain was used as the susceptible reference strain. The appropriate concentration of insecticide for bottle bioassay was determined empirically for Ae. aegypti BKK1 strain and found to be in the range of 800-1,050 μg/bottle. The time-mortality rate at 800 μg/bottle was 170±8.66 minutes, significantly different from the time-mortality rates in the 850, 900, 950, and 1,050 μg/bottle (p=0.008) concentrations, which were 135±15.00, 140±8.66, 135±15.00, and 125±8.66 minutes, respectively. The cut-off concentration selected for resistance detection was 850μg/bottle. The time-mortality rate for the Roi Et strain was 382±26.41 minutes, significantly higher than the Nonthaburi (150±25.10 minutes) and BKK1 strains (145±20.49 minutes) (p<0.001). The temephos resistance ratio (RR100) for the Ae. aegypti Roi Et strain was 2.64-fold higher at lethal time (LT100) than for the reference Ae. aegypti BKK1 strain. The mean optical density (OD) value from the biochemical microplate assay for the non-specific esterase of the Roi Et strain was higher than the mean OD for the non-specific esterase of both the Nonthaburi and BKK1 strains. Insensitive acetylcholinesterase was not found to be responsible for the resistance in the field-collected mosquitos. This study suggests that esterase detoxification is the primary cause of resistance in the Ae. aegypti population from Roi Et. Both the bottle bioassay and the biochemical microplate assay were proven to be promising tools for initial detection and field surveillance for temephos resistance.
ISSN: 01251562
Appears in Collections:Scopus 2001-2005

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