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Title: Inhibition of a conjugation-like gene transfer process in Bacillus thuringiensis subsp. israelensis by the anti-s-layer protein antibody
Authors: Chanpen Wiwat
Watanalai Panbangred
Skorn Mongkolsuk
Somsak Pantuwatana
Amaret Bhumiratana
Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Feb-1995
Citation: Current Microbiology. Vol.30, No.2 (1995), 69-75
Abstract: Extraction of the S-layer protein by treatment with 6 m urea revealed a high-molecularweight protein in the extracts obtained from Bacillus thuringiensis subsp. israelensis (B.t.i) strain 4Q2. This protein band was found to be absent in partially cured (4Q2-72) and completely cured (c4Q2-72) strains. The antibody toward this S-layer protein was prepared and used to locate its antigenic protein on B.t.i cells by using indirect immunofluorescence. Immunodiffusion reactions and Western blot analysis confirmed the specificity of the anti-S-layer protein antibody. It was found that the antibody against 4Q2 S-layer protein, inhibited plasmid transfer via a conjugationlike process between, B.t.i. strains 4Q2-16 and c4Q2-72. That is, the frequency of transfer of plasmid pBC16 was reduced from 9.7×10-6 in the absence of the antibody to less than 1.0×10-8 in the presence of the antibody. The antibody was also found to reduce the frequency of pBC16 plasmid transfer via a conjugation-like process between B.t.i. strains A084-16-194 and c4Q2-72 from 2.2×10-5 in the absence of the antibody to 1.2×10-6 in the presence of the antibody. © 1995 Springer-Verlag.
ISSN: 14320991
Appears in Collections:Scopus 1991-2000

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