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Title: Immunodiagnosis of human trichinellosis and identification of specific antigen for Trichinella spiralis
Authors: Pakpimol Mahannop
Prasert Setasuban
Nimit Morakote
Pramuan Tapchaisri
Wanpen Chaicumpa
Mahidol University
Chiang Mai University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-1995
Citation: International Journal for Parasitology. Vol.25, No.1 (1995), 87-94
Abstract: Crude antigens obtained from the infective stage larvae of Trichinella spiralis were used in an ELISA for detecting IgG antibodies to T. spiralis in serum samples collected from three groups of individuals. The individuals of the first group were parasitologically confirmed trichinellosis patients, while those of group 2 were patients with other helminthiasis and group 3 were healthy, parasite-free individuals. The specificity of the assay was 96.8% when performed on sera of groups 2 and 3. Cross-reaction was observed with the sera of patients with capillariasis, gnathostomiasis, opisthorchiasis, and strongyloidiasis and opisthorchiasis with hookworm infection. The sensitivity of the test was 100% when performed on sera of group 1, which were collected 57 days after infection. Western blot analysis revealed that a specific antigen for T. spiralis was a component of Mr109. © 1995.
ISSN: 00207519
Appears in Collections:Scopus 1991-2000

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