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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/17335
Title: Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis
Authors: Le Van Phung
Yuki Han
Shiro Oka
Hisako Hotta
Michael D. Smith
Prapit Theeparakun
Eiko Yabuuchi
Ikuya Yano
Osaka City University Medical School
Hanoi Medical University
Mahidol University
Nuffield Department of Clinical Medicine
Sappasitthiprasong Hospital
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-1995
Citation: FEMS Immunology and Medical Microbiology. Vol.12, No.3-4 (1995), 259-264
Abstract: The serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. However, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. A purified glycolipid antigen (GL) which is a specific lipid component of Burkholderia pseudomallei has been used in an ELISA. With this antigen, specific immunoglobulin G (IgG) was detected in 49 out of 50 melioidosis sera. IgG was also detected in 2 out of 185 (Japanese) and 16 out of 181 (Vietnamese) control sera. Thus, the sensitivity was 98.0%, and specificity was 98.9% and 91.1% in the Japanese and Vietnamese sera, respectively. When the ELISA and indirect haemagglutination (IHA) tests were combined, a sensitivity of 100% and specificity of 97.8% were achieved. The advantages of the glycolipid antigen are ease of preparation, stability, high sensitivity and specificity. © 1995.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0028819667&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/17335
ISSN: 1574695X
09288244
Appears in Collections:Scopus 1991-2000

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