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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/17895
Title: The effect of histological processing on the form of iron in iron-loaded human tissues
Authors: Wanida Chua-Anusorn
John Webb
David J. Macey
Pensri Pootrakul
T. G St Pierre
Murdoch University
The Institute of Science and Technology for Research and Development, Mahidol University
University of Western Australia
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 24-May-1997
Citation: Biochimica et Biophysica Acta - Molecular Basis of Disease. Vol.1360, No.3 (1997), 255-261
Abstract: Iron-loaded human spleen tissue was immersed in neutral buffered formalin over a period of 200 days. Over the first 60 days, iron leached steadily from the tissue until 3% had been lost. Thereafter, no further iron leaching was detected. Comparisons of Mossbauer spectra of freeze-dried tissue and tissue freeze-dried after immersion in formalin for 200 days showed no evidence of chemical transformation of the iron remaining in the tissue. The spectra indicated a difference in the heme-iron to non-heme iron ratio between the two samples probably reflecting inhomogeneity of the ratio throughout the spleen as measured on the centimetre scale. Mossbauer spectra of freeze-dried samples of iron-loaded human liver and pancreas tissue were compared with those for samples from the same patient that had been processed by routine hospital procedures for histology and archival. These spectra showed no evidence for chemical transformation of the iron present in the tissues. These results demonstrate that it is feasible to use archived fixed and embedded human tissue samples for studies aimed at gauging the relative fraction of goethite-like hemosiderin present in the tissue.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0030913821&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/17895
ISSN: 09254439
Appears in Collections:Scopus 1991-2000

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