Simple jQuery Dropdowns
Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/17901
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWorachart Sirawarapornen_US
dc.contributor.authorTanajit Sathitkulen_US
dc.contributor.authorRachada Sirawarapornen_US
dc.contributor.authorYongyuth Yuthavongen_US
dc.contributor.authorDaniel V. Santien_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of California, San Franciscoen_US
dc.contributor.otherThailand National Science and Technology Development Agencyen_US
dc.date.accessioned2018-07-04T07:40:50Z-
dc.date.available2018-07-04T07:40:50Z-
dc.date.issued1997-02-18en_US
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Vol.94, No.4 (1997), 1124-1129en_US
dc.identifier.issn00278424en_US
dc.identifier.other2-s2.0-0031037364en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031037364&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/17901-
dc.description.abstractSingle and multiple mutations at residues 16, 51, 59, 108, and 164 of Plasmodium falciparum dihydrofolate reductase (pfDHFR) have been linked to antifolate resistance in malaria. We prepared and characterized all seven of the pfDHFR mutants found in nature, as well as six mutants not observed in nature. Mutations involving residues 51, 59, 108, or 164 conferred cross resistance to both the antifolates pyrimethamine and cycloguanil, whereas mutation of residue 16 specifically conferred resistance to cycloguanil. The antifolate resistance of enzyme mutants found in nature correlated with in vivo antifolate resistance; however, mutants not found in nature were either poorly resistant or had insufficient catalytic activity to support DNA synthesis. Thus, specific combinations of multiple mutations at target residues were selected in nature to optimize resistance. Further, the resistance of multiple mutants was more than the sum of the component single mutations, indicating that residues were selected for their synergistic as well as intrinsic effects on resistance. Pathways inferred for the evolution of pyrimethamine-resistant mutants suggested that all multiple mutants emerged from stepwise selection of the single mutant, S108N. Thus, we propose that drugs targeted to both the wild-type pfDHFR and S108N mutant would have a low propensity for developing resistance, and hence could provide effective antimalarial agents.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031037364&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMultidisciplinaryen_US
dc.titleAntifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductaseen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1073/pnas.94.4.1124en_US
Appears in Collections:Scopus 1991-2000

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.