Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Functional interaction of a novel cellular protein with the papillomavirus E2 transactivation domain
Authors: David E. Breiding
Francis Sverdrup
Martha J. Grossel
Nicola Moscufo
Waranya Boonchai
Elliot J. Androphy
Tufts University School of Medicine
Tufts University
Harvard Medical School
Istituto Superiore Di Sanita, Rome
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-1997
Citation: Molecular and Cellular Biology. Vol.17, No.12 (1997), 7208-7219
Abstract: The transactivation domain (AD) of bovine papillomavirus type 1 E2 stimulates gene expression and DNA replication. To identify cellular proteins that interact with this 215-amino-acid domain, we used a transactivation- defective mutant as bait in the yeast two-hybrid screen. In vitro and in vivo results demonstrate that the cDNA of one plasmid isolated in this screen encodes a 37-kDa nuclear protein that specifically binds to an 82-amino-acid segment within the E2 AD. Mutants with point mutations within this E2 domain were isolated based on their inability to interact with AMF-1 and were found to be unable to stimulate transcription. These mutants also exhibited defects in viral DNA replication yet retained binding to the viral E1 replication initiator protein. Overexpression of AMF-1 stimulated transactivation by both wild-type E2 and LexA fusion to the E2 AD, indicating that AMF-1 is a positive effector of the AD of E2. We conclude that interaction with AMF-1 is necessary for the transcriptional activation function of the E2 AD in mammalian cells.
ISSN: 02707306
Appears in Collections:Scopus 1991-2000

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.