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Title: The immunoreactivity profile of different HCV genotypes on immunoblot assay and its implications in the development of diagnostic assays
Authors: Duangjit Kanistanon
Montana Neelamek
Tararaj Dharakul
Sirirurg Songsivilai
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-1997
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.15, No.3 (1997), 133-140
Abstract: The immunoreactivity profiles of plasma samples obtained from patients infected with different hepatitis C virus (HCV) genotypes were studied using immunoblot assay containing multiple HCV antigens, the immunoblot assay was found to be positive in 81.5% of 195 blood donors who had anti-HCV antibodies as detected by second generation enzyme immunoassays. The samples reacted preferentially with the viral core, NS3-1 and NS5 antigens, and these reactivities were not influenced by HCV genotype. However, the reactivities with NS3-2 and NS4 antigens varied depending on HCV genotypes. The samples from patients infected with HCV genotype 1 reacted well with NS3-2 and NS4 antigens whereas those with other genotypes did not. In addition, samples with the unclassified HCV genotype reacted poorly with all antigens, except NS3-1. This study demonstrates the importance of the core, NS3-1 and NS5 antigens in the detection of antibodies against HCV, especially in areas where more than one genotypes of HCV are present. It also demonstrates that there is a need for further improvement of the currently used assays as new HCV genotypes are recently discovered.
ISSN: 0125877X
Appears in Collections:Scopus 1991-2000

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