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dc.contributor.authorV. Viyananten_US
dc.contributor.authorE. S. Upathamen_US
dc.contributor.authorP. Sobhonen_US
dc.contributor.authorD. Krailasen_US
dc.contributor.authorP. Ardseungnoenen_US
dc.contributor.authorS. Anatawaraen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-07-04T07:46:08Z-
dc.date.available2018-07-04T07:46:08Z-
dc.date.issued1997-12-01en_US
dc.identifier.citationSoutheast Asian Journal of Tropical Medicine and Public Health. Vol.28 SUPPL. 1, (1997), 128-133en_US
dc.identifier.issn01251562en_US
dc.identifier.other2-s2.0-0031305584en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031305584&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/18015-
dc.description.abstractMonoclonal antibodies (MAbs) directed against Fasciola gigantica excretory - secretory (ES) antigens were developed from BALB/c mice. Four were selected for further study, from the panel of hybridomas. The antigen specificities of these MAbs were characterized and localized by enzyme-linked immunoeletrotransfer blot (EITB) and immunoperoxidase technique. The target epitopes of these MAbs are 66 kDa protein (MAb 2D10), 66 and 27-26 kDa proteins (MAbs 5D10 and 4F5) and 27-26 kDa protein (MAb 2D9). MAb 2D9 reacted to the antigenic components of the luminal content and epithelial cell lining the cecum, whereas MAb 2D10 reacted specifically to the antigens of the tegument and surface membrane. It was found that all MAbs cross-reacted to various degrees with the antigens extracted from Schistosoma mansoni, S. mekongi, S. spindale and Paramphistomum spp. However, when MAbs were diluted to 1:100 or 1:400 significant reduction of their cross-reactivities was observed.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031305584&origin=inwarden_US
dc.subjectMedicineen_US
dc.titleDevelopment and characterization of monoclonol antibodies against excretory-secretory antigens of Fasciola giganticaen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
Appears in Collections:Scopus 1991-2000

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