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Title: Thalassemic serum inhibits endothelial cell mitosis in vitro
Authors: Punnee Butthep
Archrob Khuhapinant
Ahnond Bunyaratvej
Suthat Fucharoen
Hiromichi Kitaguchi
Yoshinori Funahara
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Mahidol University
Kobe University School of Medicine
Keywords: Medicine
Issue Date: 1-Dec-1997
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, (1997), 155-160
Abstract: Human umbilical vein endothelial cells were cultured in vitro using Iscove's Modified Dulbecco's Medium (IMDM) supplemented with either pooled normal human serum, or pooled thalassemic serum, or autologous umbilical cord serum, or fetal bovine serum. The mitotic activity was determined under the inverted phase contrast microscope and the number of mitotic cells was counted. Our results showed that the mitotic cells decreased in endothelial cell culture with thalassemic serum as compared with normal human serum, autologous umbilical cord serum or fetal bovine serum. The percentage of mitotic cells decreased on day 3 in the presence of β-thalassemia/HbE serum from both splenectomized and non-splenectomized patients as compared with normal or autologous umbilical cord serum. In the presence of α-thalassemic serum, a similar outcome was also observed. From this study we can conclude that the thalassemic sera might contain factors which affect the endothelial cell growth and proliferation by inhibiting mitosis in vitro.
ISSN: 01251562
Appears in Collections:Scopus 1991-2000

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