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Title: Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody
Authors: Praphathip Eamsobhana
Hoi Sen Yong
Joon Wah Mak
Dorn Wattanakulpanich
Mahidol University
University of Malaya
Institute for Medical Research Kuala Lumpur
Keywords: Medicine
Issue Date: 1-Sep-1997
Citation: Southeast Asian Journal of Tropical Medicine and Public Health. Vol.28, No.3 (1997), 624-628
Abstract: A dot-blot ELISA was compared with a previously performed sandwich ELISA for the detection of Parastrongylus cantonensis antigens in sera from patients. Using the same monoclonal antibody and the same sera, 6 of 10 sera (60%) from parastrongyliasis patients were positive in dot-blot ELISA, whereas with sandwich ELISA, 5 of the same patient sera (50%) were positive. The specificity in both assays was 100% using 50 sera from patients with other parasitic diseases; of these, 10 each were from patients with cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 sera from normal healthy Thais and Malaysians. The sensitivity of the assays was, however, slightly better with dot-blot ELISA and because it is simple, quick and cost-effective, it may be a test of choice for specific diagnosis of human parastrongyliasis.
ISSN: 01251562
Appears in Collections:Scopus 1991-2000

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