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Title: Polymerase chain reaction for the detection of Burkholderia pseudomallei
Authors: Tanyachai Sura
Michael D. Smith
Gillian M. Cowan
Amanda L. Walsh
Nicholas J. White
Sanjeev Krishna
Mahidol University
Nuffield Department of Clinical Medicine
Weatherall Institute of Molecular Medicine
Keywords: Medicine
Issue Date: 1-Jan-1997
Citation: Diagnostic Microbiology and Infectious Disease. Vol.29, No.3 (1997), 121-127
Abstract: Meliodosis is a potentially lethal infection of humans and animals in Southeast Asia and northern Australia. Current methods for detection of the causative organism, Burkholderia pseudomallei, lack both speed and sensitivity. We report the development of a highly sensitive polymerase chain reaction-based method that can detect as few as 35 colony-forming units of B. pseudomallei/mL in saline suspensions. This polymerase chain reaction test also detected the presence of B. pseudomallei DNA in culture-negative splenic tissue obtained from mice infected with the organism, but without clinical evidence of disease. Specificity has been confirmed using a variety of pathogenic and nonpathogenic organisms, including B. mallei, B. cepacia, and Pseudomonas species. The clinical usefulness of this test should be assessed prospectively and compared with conventional diagnostic techniques.
ISSN: 07328893
Appears in Collections:Scopus 1991-2000

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