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Title: Characterization and expression analysis of a Xanthomonas oryzae pv. oryzae recA
Authors: Siritida Rabibhadana
Sangpen Chamnongpol
Rojana Sukchawalit
Nicholas P. Ambulos
Janine E. Trempy
Skorn Mongkolsuk
Chulabhorn Research Institute
Mahidol University
University of Maryland School of Medicine
Oregon State University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 15-Jan-1998
Citation: FEMS Microbiology Letters. Vol.158, No.2 (1998), 195-200
Abstract: Nucleotide sequence of Xanthomonas oryzae pv. oryzae (Xoo) DNA from pSM-A1 was determined and sequence analysis revealed an ORF with high homology to RecA proteins. Expression analysis using an anti-RecA antibody demonstrates that MMS treatment induces recA in trains but not in an Escherichia coli harbouring cloned Xoo recA. This indicates the existence of a recA regulatory mechanism in Xanthomonas that is not function in E. coli. In Xoo, recA was highly induced by treatments with chemical mutagens, UV and peroxides, while superoxides, a thiol agent, a heavy metal and heat shock were not inducers. The increased amount of RecA induced by H2O2or MMS treatments were due to increased transcription of recA. recA showed no growth phase or starvation regulation. The pattern of recA regulation in Xoo could play important roles in stress survival in the environment and during plant-microbe interactions.
ISSN: 03781097
Appears in Collections:Scopus 1991-2000

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