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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/18324
Title: Multiple chitinase enzymes from a single gene of Bacillus licheniformis TP-1
Authors: Srisurang Tantimavanich
Somsak Pantuwatana
Amaret Bhumiratana
Watanalai Panbangred
Faculty of Medicine, Siriraj Hospital, Mahidol University
Departments of Microbiology
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Jan-1998
Citation: Journal of Fermentation and Bioengineering. Vol.85, No.3 (1998), 259-265
Abstract: Three chitinase activity bands in a culture supernatant of Bacillus licheniformis TP-1 were detected by non-denaturing PAGE. They were designated chitinase bands 1, 2, and 3 in order from the gel origin. Analysis by immunodiffusion and immunoelectrophoresis using polyclonal antibody raised against chitinase band 3 indicated that these chitinases were antigenically similar. B. licheniformis TP-q and Escherichia coli harboring the cloned chitinase gene (pCHIL3) from strain TP-1 expressed 3 chitinase bands by non- denaturing PAGE and SDS-PAGE which gave estimated molecular masses of 68, 62, and 50 kDa (named Chi68, Chi62, and Chi50, respectively). All three chitinases had the same N-terminal amino acid sequences, strongly suggesting that Chi62 and Chi50 were derived from Chi68 by a processing step(s) at the C-terminus. The deduced C-terminal amino acid sequence of Chi68 showed homology to amino acid sequences of known chitin and cellulose binding domains. Chi62 and Chi50 lacked this domain (judging from their deduced amino acid sequences and calculated molecular masses) and they were unable to bind chitin, suggesting that they were generated from Chi68 by cleavage of the chitin binding domain at the C-terminus. Comparison of chitinase activities indicated that this binding domain was important for complete hydrolytic activity towards colloidal chitin.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031980431&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/18324
ISSN: 0922338X
Appears in Collections:Scopus 1991-2000

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